Poster presentation on research on the Discover Research Dublin night. It was a great opportunity to discuss research with both scientists and the general public.
Advanced Microscopy and Super Resolution (Andor) May 7–9 2015, Trinity College Dublin:
Lectures on the fundamentals of fluorescence
imaging including a breakdown of different types of fluorescent probes including dyes, antibodies and fluorescent proteins were
given. And a lecture on the different types of microscopes that can be used such as epi fluorescent, confocal and multiphoton and
a run through the different type of super resolution techniques such as STED and PALM were also discussed. A practical session
was run on the confocal systems in TBSI to show the basics of imaging techniques and some of the issues that arise with both
fixed and live samples.
Training on respirometry (Seahorse & Oroborus) May 5–6 2015, Trinity College Dublin.
Basic understanding of mitochondrial respiration and how the Seahorse and Oroborus systems can be used to measure mitochondrial
respiration. Demonstrations of both systems were given as well.
Basics of Fluorescence and Microscopy (ANDOR) November 6 2014, Trinity College Dublin:
A series of lectures on
camera-based systems for fluorescence imaging comparing ECCD and Cmos camera systems in terms of technology; and how
that affects the imaging quality and speed depending on experimental needs. The differences between these types of
systems and the point scanning detectors used in confocal microscopes was explored. This included a breakdown of
sensor technology, limits of imagers in terms of noise and applications versus imager choice. There were lectures on
the spinning disk technology and on Optogenetics as a new method of analysis. There were also lectures on the image
analysis software Imaris. All the above techniques with the exception of optogenetics were also demonstrated in
practical sessions.
NMR Spectroscopy Training Camp November 3–6 2014, Trinity College Dublin:
As the lead institute of the Marie Sklodowska-Curie Innovative Training Network (ITN) of TINTIN,
TCD held its first Training Camp in November 2014 at the TBSI. The area of speciality was biomolecular NMR spectroscopy
and involved the invitation of seven external and two internal academic members of staff. Along with a total of 11 lectures,
there were three practical sessions, an assessment, and a feedback session to ensure that the information/knowledge
transferred could be potentially put into practice in the real world of the ESRs.
